Figure 1.

The shedding of syndecan-1 (SDC-1), activation of STAT3 and impaired intracellular junctions induced by renal ischemia/reperfusion (I/R) injury

(A) The shedding of SDC-1 into serum was increased in the I/R mice, n = 6.

(B and C) The renal histopathological injury was aggravated in the I/R mice, Bar = 50μm, n = 6.

(D) The serum creatinine (SCr) was increased in the I/R mice, n = 6.

(E and F) The mRNA and protein expression of SDC-1 in mice kidneys from the I/R group and the Sham group, n = 6.

(G–I) Quantitative analysis of STAT3 activation and E-cadherin expression in I/R and Sham mice kidneys, n = 6–7.

(J) The histochemical staining of E-cadherin expression (n = 4) and TUNEL staining (n = 3) in the I/R and Sham mice kidneys, Bar = 50μm.

(K) The statistical analysis of TUNEL staining.

(L) The representative image of TEM for intracellular junctions between tubular epithelial cells in the I/R and Sham mice, n = 2, Bar = 1μm. ∗p < 0.05, ∗∗p < 0.01 vs. Sham group.