Figure 1.

Time course of motor–sensory impairment in MOG-induced female EAE mice. (A) EAE scoring over time demonstrated a milder progression of EAE mice having received ACF (EAE+ACF, n=18, green) at day of EAE induction when compared to untreated (EAE, n=15, black) and Echi- treated EAE animals (EAE+Echi, n=17, blue). (B) Area-under-curve (AUC) analysis confirmed partial protection from severe decline in motor–sensory function in ACF-treated EAE mice. Data are given as group mean ± SEM, and p-values were calculated using two-way ANOVA with Tukey post hoc test in (A) and Kruskal–Wallis analysis in (B). (C) Measurement of visual acuity by means of changes in cycles per degree confirmed preservation of function in ACF-treated EAE mice when compared to both EAE mice and the Echi-treated EAE group. Of note, all EAE mice demonstrated a decline in visual acuity compared to naïve control mice (n=10), and statistical differences between the naïve cohort and EAE-induced animals are displayed as § p<0.05 and §§§§ p<0.0001. The graphs in A and C display the pooled mean ± SEM per group, and the statistical difference between the EAE+ACF cohort and the untreated EAE group is indicated as *p<0.05, **p<0.01, and ***p<0.001 and as ## p<0.01 and ###p<0.001 compared to the EAE+Echi group. Analysis of visual acuity was performed using a mixed-effect model to account for repeated measurements of both eyes per sample.