Figure 3.

Effects of ACF treatment on RCG and optic nerve axon degeneration. (A) Representative images of RPBMS⁺ RCG density (upper panel) and PPD-stained optic nerve cross-sections from each EAE group indicate less degeneration in EAE+ACF mice. Analysis of RGC numbers (B) in the mid-peripheral retina, density of myelinated optic nerve axons (C), and assessment of axon damage (D) confirmed protective effects of ACF in EAE mice. All data are given as mean ± SEM, with plots representing values of each retina. Differences between groups were calculated by one-way ANOVA with Tukey post hoc test, except for D), in which differences were calculated using the Kruskal–Wallis test.