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. 2005 Mar 8;33(5):1474–1486. doi: 10.1093/nar/gki292

Figure 1.

Figure 1

Sequence and secondary structure of the HCV IRES [based on reference (60)] and beginning of the HCV-coding sequence (nt 1–510 of genotype 1a). The initiation codon of the HCV polyprotein (codon 1, nt 342–344) is highlighted. The gray arrows point to the 5′ end (nt 1) of the IRES and to the 5′end (nt 290) of the HCV insertion in the constructs without IRES (see Figure 2). The black arrows point to the 3′ end of segments of different length of the HCV-coding sequence that were used in this study. The number preceding the arrows corresponds to the last nucleotide of these segments. The sites where a +1 ribosomal frameshift was previously proposed to occur (see the text) are in bold and marked by arrowheads. The brackets indicate codons of the HCV polyprotein with their corresponding number, which are referred to in the text. The codon overlapping codon 26 in the +1 reading frame to which we also refer in the text is in bold. It was verified, using the mfold program (61), that the predicted secondary structure of the HCV sequence was not altered when it was fused to the luciferase-coding sequence.