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. 2005 Mar 8;33(5):1474–1486. doi: 10.1093/nar/gki292

Figure 5.

Figure 5

Synthesis of the polyprotein and the F protein when the AUG start codon of the polyprotein or its context is mutated. Derivatives of pHCV-447-LUC were constructed by mutating the AUG (nt 342–344) polyprotein start codon to UAC or its upstream context from ACC to UUU, generating pHCV-accUAC-LUC and pHCV-uuuAUG-LUC, respectively. Translation efficiencies are indicated on a logarithmic scale for the polyprotein and the F protein. The synthesis of the polyprotein measured with the mutant (0) constructs and that of the F protein measured with the (+1) constructs are expressed relative to the synthesis of the polyprotein with pHCV-447-LUC (0), which is arbitrarily set at 100%. Assays were made in cultured cells as described in the legend to Figure 3. Each value represents the mean ± standard error of four to six independent experiments.