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. 2005 Mar 8;33(5):1474–1486. doi: 10.1093/nar/gki292

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© The Author 2005. Published by Oxford University Press. All rights reserved

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Confirmation of the location of the translation start site of the F protein. (A) Description of the mutants used for this confirmation. A nucleotide was added in pHCV-447-LUC (+1) immediately after codon 25(+1) or 26(+1), generating pHCV+U418-LUC and pHCV+U421-LUC, respectively, where the reading frame of luciferase that monitors the synthesis of the F protein is shifted by 1 nt relative to its reading frame in pHCV-447-LUC (+1). Nt 447 was deleted (dashed line) in pHCV+U421-LUC, generating pHCV+U421ΔU447-LUC, in which the luciferase reading frame is restored. (B) Assays were made in cultured cells as described in the legend to Figure 3. The efficiency of synthesis of the F protein from construct pHCV-447-LUC (+1) is arbitrarily set at 100%. Each value represents the mean ± standard error of four to six independent experiments.