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. 2005 Mar 8;33(5):1474–1486. doi: 10.1093/nar/gki292

Figure 8.

Figure 8

Further details on the initiation of translation of the F protein. In pHCV-GCG26+1-LUC, the initiation codon of the F protein, which is GUG in pHCV-447-LUC, was mutated to GCG. In pHCV-AUG23-LUC, an AUG codon with an optimal Kozak context for initiation was introduced in the 0 frame at codon 23 of pHCV-447-LUC. In pHCV-AUG26+1-LUC, the GUG initiation codon of the F protein was mutated to AUG. In pHCV-accGUG26+1-LUC and pHCV-accGCG26+1-LUC, the Kozak context of codon 26(+1) was mutated to ACC, the initiator codon being GUG and GCG, respectively. The synthesis of the F protein is indicated on a logarithmic scale. Assays were made in cultured cells, as described in the legend to Figure 3. The synthesis of the F protein from pHCV-447-LUC (+1) is arbitrarily set at 100%. Each value represents the mean ± standard error of four to six independent experiments.