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Inhibition of the synthesis of the polyprotein and the F protein by edeine. In vitro translation experiments were carried out in 25 μl of RRL containing 0, 0.1, 0.2 or 0.3 μM edeine, with 0.2 μg of mRNAs transcribed from the FbaI-digested pHCV-LUC IRES constructs. For each construct, the protein synthesis in the absence of edeine (0 μM) is arbitrarily set at 100%. Each value represents the mean ± standard error of three independent experiments.
