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. 2023 Sep 20;299(11):105276. doi: 10.1016/j.jbc.2023.105276

Figure 2.

Figure 2

Generated M-MDSCs and TolDCs have distinct immunostimulatory and immunosuppressive capacities. Generated myeloid cells were cocultured with allogeneic CD3+ T cells for five days and shown are (A) representative histograms of CFSE-labeled pan T cells after allogeneic coculture, (B) quantified T cell proliferation and IFNγ secretion normalized to MoDC-induced response (n = 6–10). C, biplots of HLA-DR, CD86, and CD14 expression on myeloid cells in relation to induced allogeneic T cell proliferation and IFNγ secretion in the 5:1 cocultures. Generated myeloid cells were cocultured with autologous CD3+ T cells in the presence of allogeneic MoDC for five days and shown are (D) representative histograms of DC-activated CFSE-labeled T cells after autologous coculture, (E) quantified T cell proliferation, and (F) IFN γ secretion normalized to DC-activated T cell response (n = 3–5). One way ANOVA of unmatched values plus bonferroni correction in all figures except for the linear regression in HLA-DR biplots. Mean + SD are shown in all graphs ∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, ∗∗p < 0.01, ∗p < 0.05. DC, dendritic cell; MDSC, myeloid-derived suppressor cell; M-MDSC, monocyte-derived-MDSC; MoDC, monocyte-derived DC; TolDC, tolerogenic dendritic cell.