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. 2023 Sep 20;299(11):105276. doi: 10.1016/j.jbc.2023.105276

Figure 4.

Figure 4

M-MDSC and TolDC express distinct suppressive machinery. Differentially expressed immunosuppression-related molecules between M-MDSCs and TolDCs were identified by RNA microarray and mass spectrometry followed by functional validation. Shown are (A) heatmap of top 1000 variably expressed probes between M-MDSCs and TolDCs, (B) heatmap of top 500 variably expressed proteins between M-MDSCs and TolDCs, (C) expression of ROS and VSIG4 by myeloid cell types determined by flow cytometry (n = 4) and (D) secretion of IL-10 and TGF-β1 plus IDO activity determined in conditioned medium from myeloid cell types (n = 3–5). One way ANOVA of unmatched values plus bonferroni correction in all graphs. Mean + SD in all graphs ∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, ∗∗p < 0.01, ∗p < 0.05. MDSC, myeloid-derived suppressor cell; M-MDSC, monocyte-derived-MDSC; ROS, reactive oxygen species; TolDC, tolerogenic dendritic cell.