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. 2005 Mar 10;115(4):978–985. doi: 10.1172/JCI23043

Figure 3.

Figure 3

Growth inhibition and induction of apoptosis in bladder cancer cells by PLK-1 depletion using PLK-1 siRNA 1412 with cationic liposomes. (A) Cell cycle alteration of bladder cancer cells by PLK-1 siRNA 1412 (100 nM, 24 and 48 hours) was demonstrated by FACS analysis using propidium iodide staining. (B) Apoptosis induction by PLK-1 siRNA (100 nM, 36 hours) was identified by FITC–annexin V staining. Original magnification, ×100. Histogram shows the results from 3 independent experiments (500 cells each). Error bars indicate SD. (C) Comparison of growth inhibition by PLK-1 siRNA with different treatment times. PLK, cells treated by PLK-1 siRNA (100 nM); Cont, cells treated by control siRNA (100 nM). Relative cell growth was measured 72 hours after the beginning of treatments. Error bars indicate SD. Black bars, no treatment; blue bars, treated for 1 hour; gray bars, treated for 3 hours; red bars, treated for 6 hours; white bars, treated for 72 hours.