Figure 4.

Effect of SCD1 ASOs on SCD1 enzymatic activity, de novo fatty acid synthesis activity, and fat accumulation in the liver of treated C57/B6 mice on HFD. SCD1 enzymatic activity in the liver of mice treated with the various ASOs at 15 mpk for 4 weeks (A) and 10 weeks (B). The de novo fatty acid synthesis activity as indicated by the level of conversion of [³H]water into fatty acids (C) or sterols (D) in the liver tissues of mice treated with the various ASOs at 15 mpk for 10 weeks. (E–G) Images of H&E staining of liver sections of mice treated with the ASO1 (E), ASO2 (F) and ASOctrl (G) at 15 mpk for 10 weeks. The clear vacuoles in the liver section are identified by arrows in G. (H) Images of oil red O staining of frozen liver section of a mouse treated with ASOctrl at 15 mpk for 10 weeks. The lipid drops stained by oil red O within the vacuoles are identified by arrows.