Table 2.

Multivariable Cox Analyses of HOX Gene Clusters Within University Health Network, The Cancer Genome Atlas, DKFZ Cohorts.²⁷ Overall Survival Characteristics Based on Grade, G-CIMP Status, CDKN2A Status. HOX gene Cluster is Obtained Based on the Signed Average of Significant HOX Methylation Probes From Cox Analysis and Split by Median

Covariate	Multivariable Cox
	HR (95% CI)	P-value
	UHN cohort (N = 99)
HOX gene Methylation	3.86 (1.97–7.58)	8.76E-05
Grade (3 vs.2)	0.9 (0.4–2.01)	.77
Grade (3 vs. 4)	3.77 (1.3–11.4)	.019
CODEL (non-code vs. Codel)	1.21 (0.13–11.16)	.87
G-CIMP (high vs. low)	6.72 (1.73–26.15)	.006
Age	1.00 (0.96–1.03)	.88
Gender (female vs. male)	1.78 (0.91–3.49)	.09
CDKN2A (Loss vs. no loss)	2.45 (1.18–5.07)	.016
	TCGA validation cohort (N = 419)
HOX gene Methylation	2.8 (1.6–5.1)	.0005
Grade (3 vs 2)	0.5 (0.3–0.9)	.013
Grade (3 vs. 4)	1.3 (0.2–10.2)	.79
CODEL (non-code vs. Codel)	2.0 (0.8–5.1)	.16
G-CIMP (high vs. low)	1.92E–06 (0–Inf)	.997
Age	1.0 (1.0–1.1)	1.46E-05
Gender (female vs. male)	1.0 (0.6–1.7)	.88
CDKN2A (Loss vs. no loss)	3.1 (1.2–7.5)	.015
	DKFZ validation cohort (N = 211)
HOX gene Methylation	2 (1.1–3.7)	.025
Grade (3 vs. 2)	0.4 (0.3–0.7)	.0003
Grade (3 vs. 4)	0.8 (0.5–1.5)	.48
G-CIMP (high vs. low)	1.0 (0.5–2.3)	.92
Age	1.0 (1.0–1.0)	.29
Gender (female vs. male)	0.6 (0.4–0.9)	.016
CDKN2A (Loss vs. no loss)	1.1 (0.5–2.1)	.85