TABLE 1.
Bacteria and plasmids used in this study
| Bacterium or plasmid | Relevant characteristic (source or reference)a |
|---|---|
| Strains | |
| S. thermophilus MR-1C | EPS+ (21) |
| S. thermophilus TA061 | EPS− mozzarella starter (21) |
| S. thermophilus DM10 | EPS− mutant of strain MR-1C (this study) |
| L. delbrueckii subsp. bulgaricus MR-1R | EPS+ (21) |
| L. helveticus LH100 | EPS− mozzarella starter (21) |
| E. coli INVαF′ | Cloning host (Invitrogen); F′ endA1 recA1 hsdR17 (rK− mK+) supE44 thi-1 gyrA96 relA1 φ80lacZΔM15Δ(lacZYA-argF)U169λ− |
| Plasmids | |
| pSA3 | Emr shuttle vector (3, 7) |
| pCR2.1 | Kanr cloning vector (Invitrogen) |
a
Kanr, kanamycin resistance.