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. Author manuscript; available in PMC: 2024 Oct 19.
Published in final edited form as: Mol Cell. 2023 Oct 12;83(20):3692–3706.e5. doi: 10.1016/j.molcel.2023.09.024

Figure 6. Mechanism of Sen1 auto-inhibition.

Figure 6.

(A) Structural superposition of Sen1N-PP-C and the Sen1Hel RNA complex reveals a cascade of conformational rearrangements associated with autoinhibition.

(B) Surface diagram of the Sen1-RNA complex and Sen1N-PP-C shows alterations in the RNA binding channel in the closed state “C”.

(D) Fluorescence polarization ssRNA binding. Binding to ssRNA was conducted using enzyme titration and monitoring fluorescence polarization from Sub8 (Supplementary Table 3. Error bars reflect SD from 3 replicates.

(E) Superposition of an example HADDOCK docking binding pose and the autoinhibited Sen1N-PP-C state.