Key Resource Table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit Anti-MBP | GeneTex | GTX124267 |
| Mouse Anti-HA | GeneTex | GTX628489 |
| IRDye680 Goat Anti Rabbit | LI-COR | 926-68071 |
| IRDye800 Goat Anti Mouse | LI-COR | 926-32210 |
| Bacterial and virus strains | ||
| BL21-AI E. coli | Invitrogen | Cat# C607003 |
| HEK 293 Freestyle | Thermo Fisher | Cat# R79007 |
| Chemicals, peptides, and recombinant proteins | ||
| L-Arabinose | GoldBio | A-300-25 |
| Lipofectamine ™ 2000 Transfection Reagent | Thermo Fisher | 11668019 |
| Recombinant CtSen1FL (full length) | This paper | N/A |
| Recombinant CtSen1N-PP-C (1-900-PreScission-PreScission-1080-1868) | This paper | N/A |
| Recombinant CtSen1Sen1N (1-900) | This paper | N/A |
| Recombinant CtSen1Hel (1087-1890) | This paper | N/A |
| Recombinant CtSen1HelΔLZZ (1087(Δ1457-1525)-1890) | This paper | N/A |
| Recombinant CtSen1Hel-1878 (1087-1878) | This paper | N/A |
| Recombinant CtSen1Hel mutants (L1203S and L1551W) | This paper | N/A |
| Critical commercial assays | ||
| QuickChange Site-Directed Mutagenesis Kit | Agilent | 200519 |
| Q5 Mutagenesis Kit | NEB | E0552S |
| Malachite Green Phosphate Assay Kit | Cayman | 10009325 |
| Deposited data | ||
| CtSen1FL, cryo-EM map and refined coordinates | This paper | EMD: 29439, PDB: 8FTK |
| CtSen1Sen1N, cryo-EM map | This paper | EMD: 29440 |
| CtSen1N-PP-C, cryo-EM map and refined coordinates | This paper | EMD: 29426, PDB: 8FTH |
| CtSen1Hel-RNA-ADP-SO4, X-ray data and refined coordinates | This paper | PDB: 8FTM |
| Uncropped gels, Mendeley data | This paper | doi:10.17632/djm6wdc5tg.1 |
| Oligonucleotides | ||
| CtSen1Hel L1203S-fwd (AGTAAGGATCGCGGTAGTACCGAAGGCGACATC) | This paper | N/A |
| CtSen1Hel L1203S-rev (GATGTCGCCTTCGGTACTACCGCGATCCTTACT) | This paper | N/A |
| CtSen1Hel L1551W-fwd (GTTCTGTGTGCCACGTGGAGTGGCAGCGGCCAC) | This paper | N/A |
| CtSen1Hel L1551W-rev (GTGGCCGCTGCCACTCCACGTGGCACACAGAAC) | This paper | N/A |
| CtSen1Hel-1878-fwd (GTGGAAATGCACGACTAATGACGTACGAGTGAA) | This paper | N/A |
| CtSen1Hel-1878-rev (TTCACTCGTACGTCATTAGTCGTGCATTTCCAC) | This paper | N/A |
| CtSen1Hel-cHA-fwd (GCCGGATTATGCGTAATGAAAGGGTGGGCGCGC) | This paper | N/A |
| CtSen1Hel-cHA-rev (ACATCATACGGATATGGCATCGCCGGCGGGAT) | This paper | N/A |
| Substrate oligos for RNA-DNA unwinding assays | Supplementary Table 3 | N/A |
| Substrate oligos for ATPase assays | Supplementary Table 2 | N/A |
| Substrate oligos for EMSAs | Supplementary Table 3 | N/A |
| Substrate RNAs for crystallization | Supplementary Table 2 | N/A |
| Substrate RNA for fluorescence polarization RNA binding | Supplementary Table 2 | N/A |
| Recombinant DNA | ||
| CtSen1FL (full length) | This paper | N/A |
| CtSen1N-PP-C (1-900-PreScission-PreScission-1080-1868) | This paper | N/A |
| CtSen1Sen1N (1-900) | This paper | N/A |
| CtSen1Hel (1087-1890) | This paper | N/A |
| CtSen1HelΔLZZ (1087(Δ1457-1525)-1890) | This paper | N/A |
| CtSen1Hel-1878 (1087-1878) | This paper | N/A |
| CtSen1Hel mutants (L1203S and L1551W) | This paper | N/A |
| Software and algorithms | ||
| PHENIX | Adams et al.47 | N/A |
| Coot | Emsley and Cowtan45 | N/A |
| HKL-2000 | HKL Research Inc. | N/A |
| PyMOL | Schrödinger | N/A |
| Prism 8.0 | GraphPad | N/A |
| TOPAZ | Bepler et al.44 | N/A |
| Emperia Imaging Software | LI-COR | N/A |
| AlphaFold | Senior et al.46 | N/A |
| CryoSparc v2.7 | Punjani et al.42 and Punjani et al.43 | N/A |
| MotionCorr2 | Zheng et al.41 | N/A |
| Smartscope | Bouvette et al.48 | N/A |