Skip to main content
. 2023 Jul 10;120(29):e2305896120. doi: 10.1073/pnas.2305896120

Fig. 1.

Fig. 1.

(A) Engineering of chimeric Spike constructs encompassing the ectodomain and the transmembrane portion of the Wuhan-Hu-1 SARS-CoV-2 Spike with cytoplasmic tails (CT) from the following: 1. Native SARS-CoV-2 Spike; 2. no CT; 3. HIV-1 gp41, full length; 4. SIV gp41, full length; 5. HIV-1 gp41, truncated at aa. 745 of the HXB2 reference sequence; and 6. SIV gp41, truncated at aa. 745. (B) Cell-surface expression of different Spike constructs after mRNA transfection into 293-T cells. The asterisks denote statistical significance (P < 0.05) for the comparison by the two-tailed t test with each of the other conditions. (C) Schematic representation of hybrid VLPs with chimeric Spike and SIV Gag. (D) Formation of extracellular VLPs by different Spike constructs after cotransfection into 293-T cells together with SIV gag mRNA. The asterisk denotes statistical significance (P < 0.05) for the comparison by the two-tailed t test with each of the other conditions. (E) Negative-stain EM of a hybrid VLP produced with the SSt Spike.