Fig. 3.

MigPSY expression is induced during early stages of nematode infection. Relative expression levels of MigPSY genes in M. javanica following inoculation of tomato roots at the indicated time points after inoculation, as determined by qRT-PCR. Values represent relative expression levels with the level in preparasitic second-stage juveniles (J2s) set to 1. The mRNA levels were measured as three technical replicates per sample. The transcript levels of each gene were normalized to that of the nematode housekeeping gene β-actin-1 and EF-1α with three biological replicates each (n = 3). Data are presented as the mean ± SE. Each biological replicate contained a pool of hundreds of small root segments with infection sites for each post inoculation time point. dai, days after inoculation. Statistical significance was analyzed using test Holm-Sidak’s multiple comparison test. Different letters represent significant differences (P ≤ 0.05).