Skip to main content
. 2023 Jul 10;120(29):e2304612120. doi: 10.1073/pnas.2304612120

Fig. 4.

Fig. 4.

In situ hybridization of MigPSY transcripts in M. javanica and M. incognita infective juveniles. (A) Positive detection of MigPSY2 transcripts by an antisense DIG-labeled DNA probe in the subventral glands of M. javanica. (B) A sense DIG-labeled DNA probe, used as a negative control for MigPSY2 in M. javanica, showed no detectable labeling. (C) Positive detection of MigPSY3 transcripts by an antisense DIG-labeled DNA probe in the subventral glands of M. javanica. (D) A sense DIG-labeled DNA probe, used as a negative control for MigPSY3 in M. javanica, showed no detectable labeling. (E) Positive detection of MigPSY3 transcripts by an antisense DIG-labeled DNA probe in the subventral glands of M. incognita. (F) A sense DIG-labeled DNA probe, used as a negative control for MigPSY3 in M. incognita, showed no detectable labeling. Abbreviations: DIG, digoxigenin; s, stylet; m, metacorpus; SvG, subventral glands. Procedure was repeated three times independently. Representative images are shown. (Scale bars, 10 µm).