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. 2023 Jul 10;120(29):e2304612120. doi: 10.1073/pnas.2304612120

Fig. 5.

Fig. 5.

MigPSYs have a role in root-knot nematode parasitism of rice. (A) Relative transcript abundance of MigPSYs in preparasitic J2s soaked in siRNA targeting either MigPSYs or GFP. Bars represent mean ± SEM from three independent experiments (n = 3). Significant differences were assessed using Student’s t tests (two-sided; P < 0.05). (B) Number of galls present per rice root system infected with J2s soaked in siRNA targeting either MigPSYs or GFP at 30 dai. (C) Average number of females with egg masses per rice root system infected with J2s soaked in siRNA targeting either MigPSYs or GFP at 30 dai. (D) Total number of nematodes, including all developmental stages, per rice root system infected with J2s soaked in siRNA targeting either MigPSYs or GFP at 30 dai. In (B–D), approximately 1,000 M. javanica infective juveniles were inoculated onto 14-d-old rice seedlings. Experiments were performed three times independently with similar outcomes. Data from one experiment are shown. Significant differences were assessed using Student’s t tests (two-sided; P < 0.05). Source data from all three experiments are included as SI Appendix. MigPSY, M. incognita group plant peptide containing sulfated tyrosine; siRNA, small-interfering RNA; J2, preparasitic second-stage juvenile; GFP, green fluorescent protein; dai, days after inoculation; ns, not significant (P > 0.05).