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. 2023 Jul 10;120(29):e2305764120. doi: 10.1073/pnas.2305764120

Fig. 2.

Fig. 2.

CD8+ T cells are required for disease development and progression in murine AA. (A) Top 10 genes up-regulated in AA-associated CD8+ T cells compared to UG-associated CD8+ T cells in differential gene expression analysis, showing upregulation of genes associated with T cell cytotoxicity and activation such as Ccl5Gzma, and Gzmb. “0” for adjusted P value indicates a near-zero value that was rounded to 0 by the R software. (B) Top 10 GO terms enriched in AA CD8+ T cells as identified by GSEA of differentially expressed genes between AA and UG CD8+ T cells, arranged via descending Normalized Enrichment Score (NES). Shown pathways were enriched in AA CD8+ T cells in a statistically significant manner, with P < 0.05. (C) Treatment of grafted C3H/HeJ mice with anti-CD8β efficiently depleted CD8+ T cells compared to isotype control. (D) Anti-CD8β administration in grafted C3H/HeJ mice prior to hair loss onset prevented AA compared to isotype control. (E) Kaplan–Meier curve for experiment shown in (D). (F) Anti-CD8β administration in grafted C3H/HeJ mice with established disease reversed AA and induced hair regrowth. Those treated with isotype control progressed to total body hair loss. (G) Kaplan–Meier curve for experiment shown in (F). P = 0.0246.