Fig. 5.

Evolution of megalysin-like proteins. (A) Distribution of megalysin-like proteins across the tree of life. Numbers in ovals show maximum global identity with lepidopteran megalysin-like proteins. Dark blue circles indicate no known amino acid sequences with global identity >20%. Numbers in parentheses show (number of nucleotide hits with E < 1e⁻²⁰ in NCBI’s whole genome shotgun contigs database: number of WGS databases searched). Pink lines show putative horizontal gene transfer events. (B) Molecular phylogeny of megalysins and related sequence reconstructed using Bayesian inference. Numbers indicate posterior probabilities for selected nodes. The pink oval indicates posterior probability for the sister group of lepidopteran megalysin-like proteins occurring in Gammaproteobacteria.