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. 2023 Jul 12;120(29):e2305099120. doi: 10.1073/pnas.2305099120

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Copyright © 2023 the Author(s). Published by PNAS.

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Fig. 4. — Impact of vsr1 mutants on cell-type-specific gamete transcript accumulation. (A and B) Expression patterns of minus and plus gamete-specific genes in Chlamydomonas wild type (CC-5325 or CC-5155), vsr1-1 mutants, and rescued vsr1-1 mutant strains expressing HA-VSR1. Transcript levels of vegetative cells (V) or gametic cells (G) were measured using quantitative RT-PCR (qRT-PCR) with values normalized to internal control ribosomal protein gene RPL36a and then rescaled as relative expression to wild-type minus or plus gamete values, respectively. (A) minus genes GSM1 and SAD1; (B) plus genes GSP1 and SAG1. * No expression detected. (C and D) Expression patterns of Volvox GSP1 (female-specific) and GSM1 (male-specific) orthologs from qRT-PCR normalized to 18S rRNA and then rescaled to wild-type male or female values, respectively. RNA samples were derived from vegetative cultures (veg) or mature sexual cultures (sex) from indicated genotypes.