Fig. 1.

A biologically neutral OLE1 reporter. (A) Constructs to express mNeonGreen fused to the OLE1 C (Top) or N (Bottom) terminus flanked by OLE1 5′ and 3′ sequences. (B and C) Seedlings were processed for (B) immunoblotting with the indicated antibodies or (C) TLC of extracted lipids. Graphs indicate relative OLE1 signal relative to HSC70 (B) or TAG content (C) in three biological replicates (including the one shown) normalized to the WT signal at 0 d. Separate one-way ANOVA tests were performed to compare genotypes at each timepoint; letters represent homogenous subsets assigned by Tukey’s posthoc test when the P-value was <0.05. (D) Confocal images (single slices) of cotyledon epidermal cells. Neutral lipids were stained with MDH (magenta); mNeonGreen fluorescence is shown in green.