Fig. 2.

Isolation of miel1 mutants in a screen for persistent mNeonGreen–OLE1 fluorescence. (A) pOLE1:mNeonGreen–OLE1 seeds were mutagenized and their progeny was screened for sustained seedling fluorescence following germination. The Lower Right panel shows an immunoblot of 4-d-old unmutagenized pOLE1:mNeonGreen–OLE1 (WT) seedlings and miel1 mutant seedlings. (B) Representative bright field (Top) and mNeonGreen–OLE1 fluorescence (Bottom, grayscale) images of WT and miel1 mutants during germination and seedling development. (C) Gene diagram of MIEL1 showing newly identified (miel1-3 to miel1-6) and previously described (miel1-1 and miel1-2) mutations. Exons (boxes) are connected by introns (lines). Shades of purple denote MIEL1 structural domains depicted in Fig. 3.