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. 2023 Jul 6;120(29):e2304870120. doi: 10.1073/pnas.2304870120

Fig. 3.

Fig. 3.

MIEL1 is a conserved Cys-rich ubiquitin-protein ligase. (A) Alignment of MIEL1 and related proteins generated using the MegAlign (DNAStar) Clustal W method (BLOSUM series protein weight matrix). Identical residues are highlighted in black; chemically similar residues are highlighted in gray. miel1 mutations, conserved Cys and His residues, and MIEL1 domains are indicated above the sequences. (B) Phylogenetic tree including additional MIEL1 relatives constructed using the MegAlign program from the alignment shown in SI Appendix, Fig. S1. (C) Arabidopsis MIEL1 structure predicted by AlphaFold (41, 42) depicting the Zn finger-CHY (light purple), RING-H2 (medium purple), and Zn ribbon (dark purple) domains.