Fig. 4.

miel1 mutants retain oleosins, TAG, and lipid droplets longer than wild-type seedlings but do not display elevated oleosin transcript levels. (A) Relative OLE1,OLE2, and HPR transcript levels in 2-d-old seedlings measured via RT-qPCR. Letters above bars represent homogenous subsets assigned by Tukey’s posthoc test when one-way ANOVA P-values were <0.05. (B) Immunoblot probed with the indicated antibodies. Molecular weight marker positions (in kDa) are at the left. (C) TLC of extracted lipids. The graph shows relative TAG content in three biological replicates (including the one shown) normalized to the WT signal at 0 d and statistically analyzed as in the legend of Fig. 1. (D) Confocal images (single slices) of WT and miel1-6 cotyledon epidermal cells. Neutral lipids were stained with MDH (magenta); mNeonGreen–OLE1 fluorescence is shown in green. In panels B–D, 0-day samples were stratified seeds; other samples were seedlings harvested at the indicated times after sowing.