Fig. 5.
MIEL1 loss appears to decrease lipid droplet size and impact peroxisome morphology. (A) Diagram of constitutively expressed fluorescent reporters marking peroxisomal membranes (mNeonGreen–mPTSPEX26) and lumen (mRuby3–PTS1) (39). (B and C) Confocal images (single slices) of MDH-stained neutral lipids (cyan), peroxisome membranes (green), and peroxisome lumen (magenta) in cotyledon epidermal cells of 2.5- (B) and 4- (C) d-old WT, ole1-1, and miel1-5 seedlings expressing the reporters diagramed in panel A.