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MIEL1 is necessary for OLE1 ubiquitination. (A) Constructs to express HA-tagged OLE1 or ole1K-toR (with Lys residues altered to Arg) flanked by OLE1 5′ and 3′ sequences. (B) Immunoblot of proteins from 1-d-old WT seedlings expressing HA–OLE1 or HA–ole1K-toR, miel1-5 expressing HA–OLE1, or untransformed WT.
