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. 2023 Jul 6;120(29):e2304870120. doi: 10.1073/pnas.2304870120

Fig. 9.

Fig. 9.

The human MIEL1 homolog HsPIRH2 localizes to peroxisomes when expressed in Arabidopsis. (A) HsPIRH2 NMR structures (gray) (PDB 2K2C, 2JRJ, and 2K2D) (51) were aligned to the MIEL1 structure (purple) predicted by AlphaFold (41, 42). (B) Diagram of constitutively expressed fluorescent reporters marking peroxisomal lumen (tdTomato–PTS1) and human PIRH2 (mNeonGreen–HA–HsPIRH2). (C) Cotyledon epidermal cells of 4-d-old seedlings transformed with the construct shown in panel B were imaged using confocal microscopy. Maximum-intensity projections (ten 1-µm slices) of neutral lipids stained with MDH (cyan), tdTomato–PTS1 (magenta), and mNeonGreen–HA–HsPIRH2 (green) are shown. The plot shows tdTomato–PTS1 (magenta) and mNeonGreen (green) fluorescence intensity across two peroxisomes (orange arrow).