Skip to main content
. 2023 Nov 7;7(1):e202302240. doi: 10.26508/lsa.202302240

Figure 2. Mechanisms of MAC/ MACOM recruitment to nascent RNA for co-transcriptional RNA modification.

Figure 2.

The methylation complex is recruited to the nascent RNA by the following interactions/mechanisms. (A) Direct interactions between METTL14 and histone marks H3K36me3, H3K27ac, and H3K4me3 (Barbieri et al, 2017; Huang et al, 2019; Dou et al, 2023). (B) Direct interactions between WTAP and the transcription elongation factor Spt6 (Akhtar et al, 2021). (C) Direct interactions between METTL3 and histone marks H3K9me3 and H3K20me3 and maintained by m6A reader YTHDC1 (Xu et al, 2021). (D) Direct interactions between METTL14 and histone mark H3K27me3 leads to the recruitment of histone demethylase KDM6B, which directly interacts with METTL14 (Dou et al, 2023). (E) Direct interaction between the RBM15 SPOC domain with phosphorylation marks on the C-terminus of RNA polymerase II (Appel et al, 2023). (A, C, D) Note that it is not known which parts of the METTL14 (A, D) or METTL3 (C) proteins are interacting with the histone methylation marks. Structures used in this figure are as follows: METTL3/METTL14 (MAC) (PDB-ID: 5IL1), WTAP/ZC3H13/VIRMA (PDB-ID: 7VF2), and human RNA polymerase II (PDB-ID: 3J0K). ChimeraX version 1.6 was used for the visualization of experimental and predicted structures (Goddard et al, 2018; Pettersen et al, 2021).