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. 2023 Jul 10;120(29):e2303779120. doi: 10.1073/pnas.2303779120

Fig. 2.

Fig. 2.

CRY function in two discrete subsets of clock neurons can regulate rhythmicity under constant light conditions. (A–F) Actograms represent double-plotted average activity of flies from an experiment across multiple days in constant light. Flies expressing both UAS-Cas9 and UAS-3×-guides against cry (UAS-cry-g) in clock neurons are rhythmic in constant light conditions. (G) Rhythmicity index of individual flies quantified for LL2-7 represented by a boxplot, n ≥ 51 per genotype from at least two independent experiments. Letters represent statistically distinct groups; P < 0.001, Kruskal–Wallis test followed by a post hoc Dunn’s test. (H) Period under constant light for individual rhythmic flies (rhythmicity index > 0.25) quantified for LL2-7 represented by a boxplot, letters represent statistically distinct groups; P < 0.01, Kruskal–Wallis test followed by a post hoc Dunn’s test. (I and J) Phase shifts in hours in response to a 60-min light pulse at indicated times (n ≥ 26). Phase difference plotted as means ± SEM of differences from DD3-5. Same control flies are plotted in (I and J).