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. 2023 Jul 10;120(29):e2302892120. doi: 10.1073/pnas.2302892120

Fig. 3.

Fig. 3.

IFNγ signaling regulates the number of mobile microglia after microbleed in a sex-dependent manner. (A) Schematic showing timeline of experimental procedures and imaging. (B) In vivo two-photon images showing microglia movements in male mice after microbleed in wild-type (WT) controls, WT injected with IFNγ or microglia-specific knockdown of Ifngr1 (Ifngr1 KD). Mobile and stationary microglia are denoted by yellow arrow or white arrowhead, respectively. (Scale bar, 20 µm.) (C) Graphs show % of mobile microglia as a function of total cells sampled in each experimental group (MALES: WT no bleed and bleed: 119 and 145 total cells; WT+IFNγ no bleed and bleed: 105 and 252 total cells; Ifngr1 KD no bleed and bleed: 122 and 106 total cells; FEMALES: WT no bleed and bleed: 153 and 122 total cells; WT+IFNγ no bleed and bleed: 75 and 72 total cells; IFNgR1 KD no bleed and bleed: 121 and 161 total cells). Chi-square analysis was used to compare groups. (D) Bar graph shows the percentage of mobile microglia for each mouse in each experimental group. The number of mice per group is indicated in parentheses. Three-way ANOVA was used to examine main effects of Sex, Bleed, and IFNγ status. P values were derived from two-tailed Mann–Whitney tests. Data are mean ± SEM.