Fig. 2.

Western immunoblot analysis of LPS from serotype O1 representatives. The samples were prepared as whole-cell lysates. The SDS-PAGE was silver-stained, and the immunoblots were probed with the identified monoclonal and polyclonal antibodies. The difference in reactivity of the unabsorbed polyclonal Abs with the lipid A-core region in the LPS profile likely reflects known differences in outer core structures (38). The Abs were raised against a derivative of CWK2 which shares the type 1 core structure with NCTC1182 based on genome sequence (UGKQ01000007.1 and UGMM01000004.1), but B5055 has a type 2 core structure based on genome sequencing (CP072200.1) and lacks reactivity. Silver staining and western blotting were performed in triplicate with comparable results.