Fig. 5.
Comparison of catalytic effects and affinities of transition state and ground-state analogs. Distributions of catalytic and affinity effects (relative to WT PafA) for second-shell mutants with significantly deleterious catalytic effects (P < 0.05). For each plot, the two numbers denote the number of mutants with significant deleterious catalytic effects and which were measured for each parameter, and the number of mutants whose catalytic effects were measured, respectively. The solid black gridlines denote the WT value, and the dashed gridlines denote the mean fold-effect on each parameter for these mutants. The Pi affinity data are from reference (50).