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. 2023 Jul 10;120(29):e2215744120. doi: 10.1073/pnas.2215744120

Fig. 2.

Fig. 2.

Molecular characteristics of LYZ-high HCCs reveal boosted proliferation and migration. (A) Heat map shows altered pathways at the proteomic layer in paracancerous tissues (P) and HCCs distinguished by LYZ level under the optimal cut-off value. The color of each cell represents the average ssGSEA enrichment score of the indicated pathway; red denotes activation and blue denotes suppression. (B and C) GSEA of proliferation, metastasis, and metabolism-related pathways in LYZ-high HCC versus LYZ low HCC. (D) WB analysis of LYZ expression in WT and different LYZ KO HepG2 cells. Cellular β-Actin expression served as the loading control. (E) Volcano plots indicate the differentially expressed proteins in LYZ KO versus WT HepG2 cells. Red and green bubble plots represent significantly up-regulated and down-regulated proteins, respectively. Representative signature proteins associated with metabolism, proliferation, and migration are indicated. (F) GO analysis shows the biological pathways enriched by differentially expressed proteins in LYZ KO versus WT HepG2 cells. Red and blue bars denote the indicated pathways enriched by, respectively, up-regulated and down-regulated proteins. (G) GSEA plots show the indicated pathways enriched in the LYZ KO HepG2 cells.