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. 2023 Jul 10;120(29):e2215744120. doi: 10.1073/pnas.2215744120

Fig. 4.

Fig. 4.

LYZ deficiency results in decelerated cell proliferation by disrupting the cell cycle process. (A) WB analysis of LYZ expression and secretion in cell extract (CE) and culture supernatant (Sup) of HepG2 cells infected with lentivirus expressing either control shRNA (shCtrl) or LYZ-specific shRNA (sh2/sh3). (B and C) Control or LYZ knockdown HepG2 cells (B) or WT or LYZ KO HepG2 cells (C) were cultured and cell proliferation was measured at the indicated time by CCK-8. Data shown are summarized by three independent experiments. (D) WT or LYZ KO HepG2 cells were infected with either the control vector (Vec) or WT LYZ, followed by FACS sorting and WB analysis of LYZ reconstitution in these cells. Cellular β-Actin expression served as the loading control. (E) The indicated cells were cultured and cell proliferation was measured at the indicated time by CCK-8. Data shown are representative of three independent experiments. (F) FCM analysis of the cell cycle process of the indicated cells. Data shown here are summarized for three independent experiments. Data in B, C, E, and F are shown as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, n.s., no significance (two-way ANOVA followed by Tukey’s multiple comparisons).