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Oxidoreduction of iron by strains of moderately thermophilic, acidophilic bacteria grown in nonshaken cultures (100 ml of medium/250-ml conical flask) containing 25 mM ferrous sulfate, 10 mM glycerol, and 0.02% (wt/vol) yeast extract (pH 2.0) and incubated at 45°C. Symbols: •, S. acidophilus ALV; ▪, S. acidophilus THWX; ▴, S. acidophilus YTF1; ○, S. thermosulfidooxidans TH1; ▿, A. ferrooxidans TH3; +, cell-free control.
