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. Author manuscript; available in PMC: 2023 Nov 7.
Published in final edited form as: Cell Rep. 2023 Sep 19;42(9):113145. doi: 10.1016/j.celrep.2023.113145

Figure 6. Loss of EMBOW increases WDR5 and H3K4me3 levels of de novo genes.

Figure 6.

(A and B) Profile of WDR5 binding (A) or histone modification of H3K4me3 (B) on de novo WDR5 target genes in WT and EMBOW KO HEK293T cells.

(C–G) WDR5 binding and H3K4me3 levels at three de novo WDR5 target genes upon EMBOW KO shown by ChIP-seq snapshots (C) and confirmed by ChIP-qPCR with an anti-WDR5 antibody (D), an anti-KMT2A antibody (E), or an anti-H3K4me3 antibody (F). qRT-PCR results of the three target genes are shown in (G). Data represent mean values ± SEM; N = 4 biologically independent samples. Significance (p value) was evaluated with one-way ANOVA (Dunnett’s test).

**p < 0.01, *p < 0.05. Rep, replicate. See also Figure S4.