Fig. 1.

Construction of MDCK and hCK cells that overexpress B3GNT2 and/or B4GALT1. a) MDCK and hCK cells were modified with recombinant lentiviruses containing transfer plasmids for the insertion of the B3GNT2 and/or B4GALT1 gene and the Hygromycin B resistance gene. The knock-in cells were selected with 300 μg/ml Hygromycin B. b) RT-qPCR was performed with primers that anneal to both the human and dog B3GNT2, B4GALT1, or ST6GAL1 genes. The values relative to the dog GAPDH gene were used, which were then normalized to the highest value of each gene. Mean and SD (n = 3) are shown. c) Peptide MS of the B3GNT2, B4GALT1, and ST6GAL1 proteins. Only peptides unique to human proteins were selected. All samples were normalized against tubulin-β and then normalized to the highest value of each protein. Mean and SD (n = 3) are shown.