Fig. 5.

Influenza virus inoculation of B3GNT2 and B4GALT1 knock-in MDCK and hCK cells. End-point titrations with 4 control viruses and 8 recent H3N2 IAVs (details in Table 1) were performed, of which a) 4 control viruses, b) 4 3C.2a viruses, and c) 4 3C.3a viruses. Infectious titers were determined either using a hemagglutination assay a) or a NP staining b, c) when a hemagglutination assay was not possible. d) An infection study using hCK and hCK-B3GNT2 cells with a 2-fold dilution of 12 H3N2 IAVs from the 3C.2a clade (details in Table 2) that could previously either not be isolated in hCK cells (#1–8) or could be isolated in hCK cells (#9–12) was performed. Infection was assessed by the presence of cytopathic effects. Individual and mean values are shown.