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. 2023 Sep 7;8(19):e173374. doi: 10.1172/jci.insight.173374

Figure 1. YAP/TAZ are dynamically regulated during acute bleomycin lung injury.

Figure 1

(A) Schematic of injury model and time points when lungs were assessed. (B) Immunofluorescence analysis of Yap or Taz (red) in Sp-C+ (green) AT2 and Hopx+ (white) AT1 cells. (C and D) Quantification of Sp-C+ AT2 and Hopx+ AT1 cells at respective injury repair time points. (E and F) Quantification of Yap+ nuclei in Sp-C+ AT2 and Hopx+ AT1 cells. (G and H) Quantification of Taz+Sp-C+ AT2 and Hopx+ AT1 cells. Scale bars: 50 µm, 10 µm (insets). In box-and-whisker plots, whiskers are minimum and maximum, and data represent n = 6 mice. (I, J, and K) qPCR analysis of Yap/Taz target genes Axl (I), Ajuba (J), and Ctgf (K) during bleomycin injury repair. n = 6 mice for saline, 7, 14, and 21 days after injury and n = 4 mice at day 4. Data are shown as mean ± SEM. Statistical analysis was performed using 1-way ANOVA and Tukey’s post hoc comparison.