Figure 2. Deletion of YAP/TAZ leads to failed alveolar repair following single-dose bleomycin induced lung injury.

(A) Schematic of injury model in which mice were treated with tamoxifen the same day as bleomycin-induced lung injury. (B) Survival curve of WT and YT^del bleomycin- or saline-treated mice out to 28 days after injury. Statistics determined using Mantel-Cox test. (C) Masson’s trichrome staining of tissue sections from WT and YT^del mice at day 28 after bleomycin or PBS. (D) Soluble, insoluble, and total collagen quantification from WT and YT^del at day 28 after saline or bleomycin. (E) Quantification of total injured lung area in respective groups at day 28 after saline/bleomycin. n = 7 WT saline, n = 9 YT^del saline, n = 9 WT bleomycin, and n = 12 YT^del bleomycin mice. (F and G) Quantification of total Hopx⁺ cells or Sp-C⁺ cells per 20× field of view in each group. n = 10 WT saline-, n = 9 YT^del saline-, n = 12 WT bleomycin-, and n = 18 YT^del bleomycin-treated mice. (H and I) Quantification of Sp-C^tomato lineage–labeled Hopx⁺ or Sp-C⁺ AT2 cells 28 days after bleomycin. (J) Immunofluorescence analysis of Sp-C^tomato lineage labeled cells (red) and Hopx⁺ (green) AT1 cells at 28 days after injury. (K) Immunofluorescence analysis of Sp-C^tomato lineage–labeled cells (red) and Sp-C⁺ (white) AT2 cells at 28 days after injury. n = 5 WT saline, n = 5 YT^del saline, n = 10 WT bleomycin, and n = 15 YT^del bleomycin mice. Scale bars: 200 µm (C), 50 µm (J and K). Statistical analysis in D–I was performed using 1-way ANOVA and Tukey’s post hoc comparison.