Table 13.
Effect of AC3® on different parameters of reproductive/developmental toxicity study.
| Observations | Values | ||||
|---|---|---|---|---|---|
| Dosage (units) | Vehicle control | Low dose-125 mg/kg b.w | Mid dose-250 mg/kg b.w | High dose-500 mg/kg b.w | |
| Pairs started (N) | 10 | 10 | 10 | 10 | |
| Females showing evidence of copulation (N) | 10 | 10 | 10 | 10 | |
| Females achieving pregnancy (N) | 10 | 10 | 09 | 10 | |
| Conceiving days 1–5 (N) | 3 | 4 | 3 | 6 | |
| Conceiving days ≥6 (N) | 7 | 6 | 6 | 4 | |
| Pregnancy ≤21 Days (N) | 3 | 2 | 2 | 2 | |
| Pregnancy = 22 Days (N) | 6 | 7 | 6 | 7 | |
| Pregnancy ≥23 Days (N) | 1 | 1 | 1 | 1 | |
| Dams with live young born (N) | 10 | 10 | 9 | 10 | |
| Dams with live young at day 4 PP (N) | 10 | 10 | 9 | 10 | |
| Implants/dam (mean) | 11.80 | 12.50 | 11.22 | 12.60 | |
| Live pups/dam at birth (mean) | 11.20 | 11.50 | 10.56 | 11.50 | |
| Live pups/dam at day 4 (mean) | 11.10 | 11.50 | 10.44 | 11.50 | |
| Sex ratio (M/F) at birth (mean) | 105.19 | 110.05 | 104.07 | 106.92 | |
| Sex ratio (M/F) at day 4 (mean) | 102.69 | 110.05 | 102.08 | 106.92 | |
| Litter weight at birth (mean) | 69.90 | 72.83 | 63.61 | 75.07 | |
| Litter weight at day 4 (mean) | 84.23 | 95.07 | 78.63 | 98.12 | |
| Pup weight at birth (mean) | 6.24 | 6.33 | 6.03 | 6.53 | |
| Pup weight at the time of AGD measurement (mean) male/females | M | 7.61 | 8.17 | 7.69 | 8.48 |
| F | 7.55 | 8.37 | 7.57 | 8.59 | |
| Pup AGD on the same postnatal day, birth–day 4 (mean male/females note postnatal day) | M | 2.84 | 2.85 | 2.88 | 2.83 |
| F | 1.82 | 1.84 | 1.86 | 1.83 | |
| Pup weight at day 4 (mean) | 7.59 | 8.27 | 7.58 | 8.53 | |
| Male pup nipple retention at day 13 (mean) | 0 | 0 | 0 | 0 | |
| Pup weight at day 13 (mean) | 18.72 | 19.09 | 19.73 | 19.91 | |
| Abnormal pups | |||||
| Dams with 0 (N) | 10 | 10 | 9 | 10 | |
| Dams with 1 (N) | 0 | 0 | 0 | 0 | |
| Dams with ≥2 (N) | 0 | 0 | 0 | 0 | |
| Loss of offspring | |||||
| Prenatal/postimplantations (implantations minus live births) | |||||
| Females with 0 | 6 | 3 | 7 | 7 | |
| Females with 1 | 3 | 4 | 1 | 1 | |
| Females with 2 | 0 | 3 | 1 | 2 | |
| Females with ≥3 | 1 | 0 | 1 | 0 | |
| Postnatal (live births minus alive at postnatal day 13) | |||||
| Females with 0 | 9 | 10 | 9 | 10 | |
| Females with 1 | 1 | 0 | 1 | 0 | |
| Females with 2 | 0 | 0 | 0 | 0 | |
| Females with ≥3 | 0 | 0 | 0 | 0 | |
M: male; F: female; N: number; PP: postpartum; AGD: anogenital distance.