Table 2.

IR absorption band changes and possible assignment for the metal-free and metal-loaded Klebsiella strain sp. R3.

FTIR peak	Klebsiella sp. strain R3
	Metal-free	Metal-loaded	Displacement*	Functional groups	Bond	Assignment
1		667	667	C2H2R2	C-H out-of-plane-bend	Alkene
2	719	712	7	1,3-Disubstituted (Aromatic compounds)	C-H out-of-plane-bends	Aromatic
3	738	742	4	1,2-Disubstituted (Aromatic compounds)	C-H out-of-plane-bend	Aromatic
4	805	798	7	C2HR3	C-H out-of-plane-bend	Alkene
5		846	846	1,4-Disubstituted (Aromatic compounds)	C-H out-of-plane-bend	Aromatic
6		876	876	1,3-Disubstituted (Aromatic compounds)	C-H out-of-plane-bend	Aromatic
7		1,003	1,003	(RCO)2O	C-O stretch	Carbonyl
8		1,128	1,128	R-OH	C-O stretches	Alcohol
9		1,282	1,282	RCOOR’	C-O stretch	Carbonyl
10	1,457	1,469	12	C-C	C-C bend	Alkane
11	1,558	1,557	1	P-NH2	NH2	Amine
12	1,647	1,640	7	R2C=NR or R2C=NH	C=N stretch	Imine and Oxime
13		1,700	1,700	R2C=O or RCOOH	C=O stretch	Ketone or carboxylic acid
14		1,718	1,718	R2C=O or RCOOH	C=O stretch	Ketone or carboxylic acid
15		1,770	1,770	RCOC1	C=O stretch	Acid Chloride
16	2,121	2,117	4	C ≡ C	C ≡ C stretch	Alkyne
17	2,147	2,136	11	C ≡ C	C ≡ C stretch	Alkyne
18		2,162	2,162	C ≡ C	C ≡ C stretch	Alkyne
19	2,177	2,184	7	C ≡ C	C ≡ C stretch	Alkyne
20	2,195	2,199	4	C ≡ C	C ≡ C stretch	Alkyne
21	2,251	2,259	8	RC ≡ N	C ≡ N stretch	Nitrile
22	2,859	2,861	3	C-H	C-H stretch	Alkane
23	2,986	2,984	2	P-NH	NH	Amine
24		3,120	3,120	C=C-H	C-H stretch	Alkene
25		3,224	3,224	P-NH2	NH2	Amine
26		3,280	3,280	P-NH2	NH2	Amine
27		3,493	3,493	RO-H hydrogen bond	O-H stretch	Hydroxyl
28		3,993	3,593	RO-H free	O-H stretch	Hydroxyl
29	3,630	3,623	7	RO-H free	O-H stretch	Hydroxyl

*IR band shifts in red; new bands in blue.