Table 3.

IR absorption band changes and possible assignment for the metal-free and metal-loaded Klebsiella sp. strain R19.

FTIR peak	Klebsiella sp. strain R19
	Metal-free	Metal-loaded	Displacement*	Functional groups	Bond	Assignment
1		675	675	C2H2R2	C-H out-of-plane bend	Alkene
2	716	719	3	1,3-Disubstituted (Aromatic compounds)	C-H out-of-plane bend	Aromatic
3	734	737	4	Monosubstituted	C-H out-of-plane bend	Aromatic
4		768	768	1,2-Disubstituted (Aromatic compounds)	C-H out-of-plane bend	Aromatic
5	801	813	12	C2HR3	C-H out-of-plane-bend	Alkene
6		846	846	1,4-Disubstituted (Aromatic compounds)	C-H out-of-plane-bend	Aromatic
7		924	924	P-NH2	NH2	Amine
8		939	939	P-NH2	NH2	Amine
9	962	964	2	C2H2R2	C-H out-of-plane-bend	Alkene
10		988	988	C2H3R	C-H out-of-plane-bends	Alkene
11		1,118	1,118	R-OH	C-O stretches	Alcohol
12	1,230	1,238	8	R-OH	C-O stretches	Alcohol
13	1,368	1,373	5	R-NO2	N=O	Nitro
14	1,390	1,393	3	R-NO2	N=O	Nitro
15	1,435	1,434	1	C-C	C-C bend	Alkane
16	1,457	1,461	4	C-C	C-C bend	Alkane
17	1,487	1,490	2	C=C	C=C	Aromatic
18		2,147	2,147	C ≡ C	C ≡ C stretch	Alkyne
19	2,166	2,162	4	C ≡ C	C ≡ C stretch	Alkyne
20	2,188	2,193	5	C ≡ C	C ≡ C stretch	Alkyne
21		2,210	2,210	C ≡ C	C ≡ C stretch	Alkyne
22		2,230	2,230	C ≡ C	C ≡ C stretch	Alkyne
23		2,232	2,232	C ≡ C	C ≡ C stretch	Alkyne
24	2,837	2,840	3	RCHO	C-H stretch	Aldehyde
25	2,881	2,889	8	C-H	C-H stretch	Alkane
26		2,911	2,911	C-H	C-H stretch	Alkane
27		2,930	2,930	C-H	C-H stretch	Alkane
28	2,926	2,960	34	C-H	C-H stretch	Alkane
29		2,986	2,986	P-NH	NH	Amine
30		2,997	2,997	P-NH	NH	Amine

*IR band shifts in red; new bands in blue.