Figure 3.

The CD49b⁺CD229⁺ subset lacks extensive long-term reconstituting ability. (A) Leukocyte repopulation in the peripheral blood (PB) of secondary transplanted mice from CD49b^–CD229^–, CD49b^–CD229⁺, CD49b⁺CD229^–, and CD49b⁺CD229⁺ populations. (B) Donor-derived myeloid, platelet, erythrocyte, B cell, and T-cell frequency in the PB of secondary transplanted mice. n_CD49b^–_CD229^– = 9 mice, n_CD49b^–_CD229⁺ = 6-7 mice, n_CD49b⁺_CD229^– = 5 mice, and n_CD49b⁺_CD229⁺ = 4-5 mice, 1 experiment, in (A-B). (C) Proportion of secondary transplanted mice with ≥0.1% donor-derived myeloid cells in the PB, 6 months post-transplantation. n_CD49b^–_CD229^– = 8 mice, n_CD49b^–_CD229⁺ = 6 mice, n_CD49b⁺_CD229^– = 5 mice, and n_CD49b⁺_CD229⁺ = 4 mice, 1 experiment. (D) Estimated HSC frequency in secondary transplantation, based on limiting dilution calculation from Fig. 2H and the number of mice with active LT myeloid reconstitution from Fig. 3C. Data are represented as mean ± SD in (A-B).