Fig 6.

Anti-inflammatory effects of the isolated compounds from MESD in LPS-induced RAW 264.7 cells.

(A, B) Nitric oxide (NO) and Prostaglandin E₂ (PGE₂) content in cell culture media in LPS-induced RAW 264.7 cells. (C, D) Protein expression and quantitative analysis of iNOS and COX-2 in RAW 264.7 cells treated with the isolated compounds (40 µM and 80 µM). (E, F) mRNA levels of interleukin-1β (Il-1β) and interleukin-6 (Il-6) in LPS-induced RAW 264.7 cells. All data are presented as mean ± SEM, with significant differences at p <0.05. (prim-O-glucosylcimifugin (1), cimifugin (2), 4′-O-β-d-glucosyl-5-O-methylvisamminol (3), 5-O-methylvisamminol (4), 3′-O-acetylhamaudol (5), deltoin (6), and 3′-O-angeloylhamaudol (7)).