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. 2023 Nov 2;20(11):1769–1779. doi: 10.1038/s41592-023-02040-5

Fig. 4. CINEMA-OT identifies a heterogeneous defensive response in human airway epithelial cells exposed to rhinovirus and cigarette-smoke extract.

Fig. 4

a, Overview of experimental design. Differentiated airway epithelial organoids are challenged with mock (control) or RV infection, with or without CSE exposure. b, UMAP projection of expression data labeled by perturbation and cell type. c, UMAP projection of the individual treatment-effect matrix obtained by CINEMA-OT from the RV response without CSE exposure, colored by response cluster. d, Gene set enrichment analysis of mock to RV response clusters identified by CINEMA-OT, colored by adjusted P value. e, UMAP projection of the individual treatment effect matrix obtained by CINEMA-OT from the CSE response without RV exposure, colored by response cluster. f, Gene set enrichment analysis of mock to CSE response clusters identified by CINEMA-OT, colored by adjusted P value. g, Cell-wise synergy score visualization. h, UMAP visualization of CINEMA-OT synergy embedding, colored by cell type. f, Two diverging patterns among CINEMA-OT identified synergistic genes, visualized by stacked violin plots and dot plots.