Table 2.
GO Functional enrichment and pathway enrichment analysis of differentially expressed miRNA target genes
| GO | ||||
|---|---|---|---|---|
| Term | Category | P value | UniProt ID | |
| BP | ||||
| Positive regulation of transcription by RNA polymerase II | GO:0045944 | 3.61113E−33 | DLX6, ETV1, PAX6, TFAP2B, PAX7, DCN, CALCOCO1,SLC30A9, HGF, IKZF2, | |
| Regulation of transcription, DNA-templated | GO:0006355 | 1.55795E−28 | NFYA, ZFX, ZNF263, DLX6, ETV1, PAX6, TFAP2B, PAX7, ZNF207, SCMH1 | |
| Multicellular organism development | GO:0007275 | 2.13391E−30 | WNT16, DLX6, IFRD1, GAS7, PAX6, SCMH1, FYN, BTBD7, SPAST, MUSK | |
| Negative regulation of transcription by RNA polymerase II | GO:0000122 | 5.32813E−28 | ZNF263, PAX6, TFAP2B, STRAP, CUL3,HDAC9, NEDD4L, MXD1, MXD1, HIPK2 | |
| Cell cycle | GO:0007049 | 3.95899E−26 | KMT2E, RALA, DBF4, ZNF207, TTC19, SPAST, CUL3, BOD1L1, SPDL1, CTDP1, | |
| Positive regulation of transcription, DNA-templated | GO:0045944 | 3.61113E−33 | DLX6, ETV1, PAX6, TFAP2B, PAX7, DCN, CALCOCO1, SLC30A9, HGF, IKZF2 | |
| Nervous system development | GO:0007399 | 2.16476E−23 | DLX6, KDM7A, GAS7, TENM1, SPAST, GPM6B, ARID1B, NEXMIF, CAMK2B, MEF2A | |
| Cell differentiation | GO:0030154 | 3.59095E−21 | ABCB5, DLX6, ETV1, IFRD1, GAS7, PAX6, FYN, SPAST, GLRX2, MUSK | |
| Phosphorylation | GO:0016310 | 1.52377E−20 | AK2, MAP3K14, CDKL5, FYN, MAP4K3, MUSK, EPHA3, MAPK9, PIK3CB,EIF2AK2 | |
| Angiogenesis | GO:0001525 | 1.73547E−19 | NOX1, NRXN3, CALCRL, RORA, TGFBR3, CEACAM1, NRCAM, ANGPT2, NRP1, POFUT1 | |
| MF | ||||
| Protein binding | GO:0005515 | 2.9008E−264 | GCLC, NFYA, NIPAL3, ANKIB1, HECW1, LASP1, M6PR,CFLAR, VPS50, ST7 | |
| Metal ion binding | GO:0046872 | 1.37913E−65 | ANKIB1, LASP1, KMT2E, ITGAL, ZFX, ZNF263, KDM7A, DBF4, CACNA2D2, NOX1 | |
| Transferase activity | GO:0016740 | 3.73593E−37 | ANKIB1, HS3ST1, HECW1,AK2, MAP3K14, CDKL5, UBE3C, REV3L, POMT2, FYN | |
| DNA binding | GO:0003677 | 1.59763E−34 | NFYA, ZNF263, ZFY, DLX6, ETV1, PHTF2, PAX6, TFAP2B, REV3L, PAX7 | |
| DNA-binding transcription factor activity, RNA polymerase II-specific | GO:0000981 | 6.52672E−25 | NFYA, ZFX, DLX6, MNT, ETV1, PAX6, TFAP2B, PAX7, MXD1, FOXJ2 | |
| RNA polymerase II cis-regulatory DNA binding region sequence-specific DNA binding | GO:0000978 | 1.02216E−24 | NFYA, DLX6, ETV1, PAX6, TFAP2B, CALCOCO1, MXD1, FOXJ2, NFYC, MEF2A | |
| Nucleotide binding | GO:0000166 | 2.04842E−23 | ASNS, STK10, MAP2K5, CDK13, NAIP, STRADB, EIF2AK1, SLC22A4, REM1, OAS1 | |
| Kinase activity | GO:0016301 | 9.56582E−20 | AK2, MAP3K14, CDKL5, FYN, MAP4K3, MUSK, EPHA3, MAPK9, PIK3CB, EIF2AK2 | |
| Sequence-specific DNA binding | GO:0043565 | 3.36805E−19 | ZNF263, DLX6, ETV1, PAX6, TFAP2B, PAX7, CALCOCO1, FOXJ2, TP53BP1, CBFB | |
| DNA-binding transcription factor activity | GO:0003700 | 1.97388E−18 | NFYA, ZNF263, DLX6, ETV1, PAX6, TFAP2B, PAX7, ZNF207, WIZ, IKZF2 | |
| CC | ||||
| Cytoplasm | GO:0005737 | 3.5946E−152 | ANKIB1, WNT16, HECW1, LASP1, CFLAR, AK2, CDC27, KMT2E, IBTK, MAP3K14 | |
| Cytosol | GO:0005829 | 5.06695E−97 | GCLC, HECW1, CFLAR, VPS50, CDC27, MAP3K14, AP2B1, FARP2, PAX6, SYN1 | |
| Nucleus | GO:0005634 | 1.93966E−86 | NFYA, CDC27, KMT2E, IBTK, ZFX, ZNF263, DLX6, KDM7A, ETV1, PHTF2 | |
| Cytoskeleton | GO:0005856 | 1.21282E−47 | LASP1, KMT2E, FARP2, GAS7, SYN1, CDKL5, TENM1, ZNF207, SLC30A9, SPAST | |
| Nucleoplasm | GO:0005654 | 5.30388E−51 | NFYA, CDC27, KMT2E, IBTK, ZFX, MAP3K14, KDM7A, DBF4, PAX6, TFAP2B | |
| Cytoplasmic vesicle | GO:0031410 | 1.81706E−27 | LAMP2, SPAG9, IYD, HFE, SLC30A9, SPAST, GABRA1, VEZT, SLC4A7, SLC18A1 | |
| Cell projection | GO:0042995 | 6.32442E−27 | NOX1, CDKL5, ADAM22, EHD3, CD44, VEZT, SNX1, IFT88, SLC4A7, CUL3 | |
| Neuronal cell body | GO:0043025 | 4.53325E−22 | TAC1, GLRX2, CASR, USH2A, LRP6, CDC42, TRPC5, FBXW11, MAP2, CHRNA3 | |
| Mitochondrion | GO:0005739 | 1.13123E−20 | GCLC, AK2, ABCC8, FYN, TTC19, LARS2, GLRX2, ADSS2, TRIT1, HSPA5 | |
| Centrosome | GO:0005813 | 1.85671E−20 | CDC27, CDKL5, TTC19, SPAST, RRAGD, IFT88, LRRC7, CUL3, BOD1L1, OFD1 | |
| KEGG | ||||
| PI3K-Akt signaling pathway | hsa04151 | 4.41E−16 | KITLG, PIK3CB, PPP2R5A, GNB5, PPP2R3A, ITGA8, COL4A4, ANGPT2, JAK2, MAPK1, FLT1, SGK3, IL7, CDK6,RPS6KB1,NFKB1, CDKN1B, LAMA4, GHR, PRLR | |
| Focal adhesion | hsa04510 | 1.49265E−09 | PPP1CB, FYN, HGF, BIRC3, VCL, MAPK9, PIK3CB, MYLK, ROCK1, CDC42, ACTN1, PAK3, ITGA8, COL4A4, MAPK1, FLT1, CAV2, MAPK8, MAPK10, BIRC2 | |
| Calcium signaling pathway | hsa04020 | 7.41132E−11 | PHKA2, PTGER3, CAMK2B, ATP2B4, MYLK, PHKA1, ATP2B1, P2RX7, SLC8A3, PHKB, STIM2, ADRA1A, ADCY7, PTGFR, ITPR2, PLCG1, CHRM3, LHCGR, PLCE1, PPP3CA | |
| JAK-STAT signaling pathway | hsa04630 | 2.08628E−09 | IFNGR1, PIK3CB, PIAS2, JAK2, IL7, GHR, IL12B, PRLR, LIFR, STAM2, AKT3, PIK3CA, IL13RA1, RAF1, STAM, IFNA21, EGF, EGFR, IFNAR2, JAK1 | |
| Pathways in cancer | hsa05200 | 1.20E−23 | WNT16, RALA, HGF, BIRC3, FAS, IFNGR1, KITLG, PTGER3, MAPK9, PIK3CB, CAMK2B, ROCK1, GNB5, LRP6,DC42, WNT8B, PLD1, MLH1, ARAF, RUNX1T1 | |
| Rap1 signaling pathway | hsa04015 | 5.98534E−14 | ITGAL, RALA, FARP2, HGF, KITLG, PIK3CB, CDC42, GNAO1, ANGPT2, MAPK1, FLT1, MAPK14,PRKD3, RAP1A, AKT3, CNR1, FGF23, ADCY7, PIK3CA, PLCG1, | |
| Axon guidance | hsa04360 | 2.09295E−13 | CRKL, FYN, EPHA3,PIK3CB, CAMK2B, ROCK1, CDC42, TRPC5, SEMA3A, PAK3, SSH1, SEMA6A, NRP1, MAPK1, FZD3, UNC5A, WNT5A, EPHA4, PIK3CA, PLCG1, | |
| Ras signaling pathway | hsa04014 | 5.20834E−12 | RALA, HGF, KITLG, MAPK9, PIK3CB, GNB5, CDC42, PLD1, PAK3, BRAP, ANGPT2, MAPK1, FLT1, MAPK8, RAB5C, NFKB1, MAPK10, GAB1, RAB5B, EXOC2 | |
| cAMP signaling pathway | hsa04024 | 2.06924E−10 | PTGER3, MAPK9, PIK3CB, CAMK2B, ATP2B4, PDE4A, ROCK1, ATP2B1, PLD1, ADCYAP1R1, MAPK1, MAPK8, NFKB1, MAPK10,PDE4D, RAP1A, AKT3, CREB1, GRIA2, ADCY7 | |
| Hippo signaling pathway | hsa04390 | 1.8018E−09 | WNT16, MPP5, FBXW11, WNT8B, DLG1, AFP, AXIN1, FZD3, TGFBR1, WNT3, BIRC2, BMP5, WNT5A, BMP2, DLG4, PATJ, CSNK1D, WNT9A, TCF7L2, PPP2R2B | |
Category refers to the pathway functional categories